Qingdao Energy Institute made progress in biotransformation of Jerusalem artichoke

Jerusalem artichoke, also known as Jerusalem artichoke, belongs to non-food crops, and its soil adaptability is strong. It can be planted in non-cultivated marginal land such as drought and saline. Jerusalem artichoke tubers are rich in inulin and inulin is the second largest storage polysaccharide in plants, second only to starch. The development of the starch sugar industry has become more mature, and the development and application of inulin remains to be developed. With inulin as a sugar platform, combined with modern biotechnology, bioenergy, bio-based materials, medicine and food can be developed.

Recently, the microbial resources team of the Institute of Bioenergy and Bioprocess Research of the Chinese Academy of Sciences in Qingdao focused on the conversion of inulin to fuel ethanol, an energy product, and fructose, a functional food “prebiotic”, and made progress. In the previous study, the researchers established an ethanol biosynthesis process for Jerusalem artichoke, which integrates the inulinase production, inulin hydrolysis, and ethanol fermentation into a single process that enables the direct fermentation of Jerusalem artichoke to produce fuel ethanol. The yeast fermentation at 200°C at 40°C. L Jerusalem artichoke powder, ethanol production 65.2 g / L, ethanol yield was 79.7%. The team has effectively improved the conversion efficiency of Jerusalem artichoke through the transformation of yeast polysaccharide metabolic pathways and enzyme secretion systems, fermenting 250 g/L of Jerusalem artichoke powder, producing 81.8 g/L of ethanol, a yield of 3.13 g/L/h, and a conversion rate of up to 92%. Related research results were published on Biotechnology for Biofuels.

The fructo-oligosaccharide products are mainly converted from sucrose by enzymatic conversion. The theoretical conversion rate of this process is less than 60%. The production of high-purity fructooligosaccharides requires purification and increases costs. Inulin is catalyzed by inulinase to produce fructo-oligosaccharides, but inulinase activity is usually not high, and currently no cheap commercial enzymes are available. The researchers established a simple process for the production of high-purity fructo-oligosaccharides by yeast fermentation by expressing inulinase enzymes in Saccharomyces cerevisiae and eliminating the ability of the strains to metabolize the inulin components. Using this process, 200 g/L inulin was fermented at 40° C. to produce fructooligosaccharides 180 g/L with a yield of up to 7.5 g/L/h. The relevant research results were published on Carbohydrate Polymers and have applied for a Chinese invention patent (Patent No. 2016103904308).

The above technical inventions will promote the development of Jerusalem artichoke products and promote the development of the Jerusalem artichoke industrial chain.

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